Response and Persistent Contamination Levels in Estuarine Fish
A. Maruya (formerly: Skidaway Institute of Oceanography, Savannah, Georgia, USA)
Sea Grant College Program (R/HAB-10-PD).
3/01/2000 - 2/28/2002 (complete)
Genotoxic contaminants threaten the integrity of estuarine ecosystems; robust,
sensitive and non-destructive indicators of genotoxicity in large organism,
particularly, fish, are lacking. Previous research has no agreed whether FIBCOT
is immune to other (non-contaminant) environmental stressors. Development of
FIBCOT will help coastal resource professionals assess the impacts of persistent,
genotoxic contaminants on the health of wild fish populations, without interference
from other environmental stressors. The objectives of this project were:
- To adapt and
characterize the response of an assay that measures DNA damage in fish blood
as an indicator of genotoxicity.
- To characterize
the effect on non-chemical stressors on the fish blood comet assay (FIBCOT).
The Comet assay was adapted and analytical conditions were optimized for whole
blood of 2 fish species (Fundulus and seatrout). It was determined that FIBCOT
was relatively insensitive to changes in several, non-contaminant environmental
parameters, including water temperature, pH, salinity, and dissolved oxygen
of Phenolic and Dioxin-like Contaminants on Coastal Georgia Water Quality
for the Georgia Coast